Optimization of Nutrient Media of in Vitro Propagation for Some Cherry Rootstocks (Prunus Cerasus)

Authors

  • Avdirrahman Gashi Faculty of Agricultural & Environment, Agricultural University of Tirana
  • Tokli Thomaj Faculty of Agricultural & Environment, Agricultural University of Tirana
  • Taulant Mitrushi Faculty of Agricultural & Environment, Agricultural University of Tirana
  • Edlira Kukali Faculty of Agricultural & Environment, Agricultural University of Tirana

Abstract

The most important species of which have origin many populations, land-races and local varieties: wild cherry, wild pears, wild almond, wild medlar, etc. This study was focused in many important rootstocks of P. avium x P canascens - Gisela 6, P.avium x P. pseudocerasus - Colt (nano), P. mahaleb x P.avium (Maxma), P. Mahaleb and cultivar Prunus avium L. (Cerasus avium L. var. sativa Ser., P. cerasus): Bigarreau Hatif. Are used two important media in the micro propagation of the horticultural species above, MS (Murashige & Skoog) and LP (Quoirin & Lepoivre). Nutrients of MS media have affected with sensible changes compare with LP media, in the explants proliferation for differentiations of the buds, shoots and leafs. Inoculation of explants in LP media, results with lower result on the qualitative indicators of the research. Average growing of the shoots on the MS media, resulted 10.1 mm while on the LP media was 6.0 mm. Average number of leafs 2.47 on the MS media, while on the media LP was 1.34 leaf. In the statistic analyses for the min and max, in all the cases it has predominance of the MS media. Intensity of the growing has cv (coefficient of variation) = 63% and on the MS media results 1.88 highest of LP media.

Keywords:  Murashige & Skoog, Quoirin & Lepoivre, buds, leafs, shoots.

References

Aitken-Christie J., Kozai T., Takayama S, (1995). Automation in plant tissue culture General introduction and overview. In: Automation and environmental control in plant tissue culture (eds. Aitken-Christie J., Kozai T., Smith M. L.). Kluwer Academic Publishers, Dordrecht, the Netheland.

M.Akita, K.Negishi, A.Kitano, M.Wasaki, Y.Ohta, T.Kuriu, and T.Takii, . (2006), Acta Horticulture, 579-584.

G.M.Bonga, P.V.Adkares; (2004). In vitro culture of trees, 1st edition. Trans. by E.Bagheri, et al., Ferdowsi University Press, Mashhad, pp.96-104.

A.Carolina, M.Paula, C.Pijut, H.Michler, Horticulture Science. 2006, 41(1), 193-201.

Murashige T., Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15(3): 473-497.

E. Kukali, E. Kongjika, (2011). Journal of Environmental Protection and Ecology , ISSN 1311-5065, Book 1, factor Impact 0.33; The Importance of genetic material ‘In Vitro’, rizogenic ability of grape

Nacheva L., Gercheva P. (2009). Micropropagation of ‘Gisela 5’ (cherry dwarf rootstock): The effect of the type and the concentration of the carbohydrates in the nutrient medium. Acta Horticulturae 825: 261-267. DOI: 10.17660/ActaHortic.2009.825.41.

Pierik R.L.M. (1997). In vitro culture of higher plants. Springer Science and Business Media, Springer, Dordrecht, the Netherlands. DOI: 10.1007/978-94- 011-5750-6.

Quoirin M., Lepoivre P. (1977). Edude de milieu adaptes aux cultures in vitro de prunus. Acta Horticulturae 78: 437-442 (in French).

Sœbø A., Krekling T., Appelgren M. (1995). Light quality effects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell, Tissue and Organ Culture 41, 177 – 185.

Solarova J., Souckova D., Ullmann J., Pospisilova J., (1996). In vitro culture: environmental conditions and plantlet growth as effected by vessel and stopper type. HortScience 23(2), 51–58.

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Published

2018-01-01

How to Cite

Gashi, A., Thomaj, T., Mitrushi, T., & Kukali, E. (2018). Optimization of Nutrient Media of in Vitro Propagation for Some Cherry Rootstocks (Prunus Cerasus). ANGLISTICUM. Journal of the Association-Institute for English Language and American Studies, 6(12), 54–61. Retrieved from https://www.anglisticum.org.mk/index.php/IJLLIS/article/view/1591

Issue

Vol. 6 No. 12 (2017): Volume 6, No.12, December, 2017

Section

Volume 6, No.12, December, 2017

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