Isolation of C. Sakazakii and Concentration of Enterobacteriaceae in Kitchen’s Neonatal Intensive Care (NIC)
Abstract
International Commission for Microbiological Specification for Foods ranked Cronobacter sakazakii as "Severe hazard for restricted populations, life-threatening or substantial chronic sequelae or long duration" (ICMSF, 2002). Among infants at greatest risk are infants less than 2 months of age. This research aimed to get more insight the habitat, to control the biological risk of the hospital kitchen's environment at the University Hospital of Obstetrics and Gynaecology "Queen Geraldine", where the powered infant formula is prepared. Efficiency of risk control must be verified through the application of microbiological monitoring plan that provides application of microbiological environmental criteria, the proper cleaning of equipment used in production lines, control of the final product during their shelf live, collection of samples from the raw material, surfaces and environment as well as to take measures during preparation and reconstitution of powered infant formulae. This study was performed to determine the frequency distribution of C. sakazakii and the concentration of Enterobacteriaceae in different sampling areas of the kitchen. In order to identify potential environmental reservoirs we have analyzed 60 samples from the kitchen areas as well as personal hands. At the end, we have quantified Enterobacteriaceae and isolate C. sakazakii from the total of 60 samples. We have detected the presence of C. sakazakii in two environmental samples (3.3%) of the cases. Care should be taken while preparing storing and handling PIF and rules should be respected to meet the highest level of microbiological safety in hospital/nursery as defined in MRA Series 10 (FAO/WHO 2004).
Keywords: PIF, Cronobacter sakazakii, food safety, Enterobacteriaceae.
Downloads
Published
How to Cite
Issue
Section
License
Declaration/Copyright transfer:
1. In consideration of the undertaking set out in paragraph 2, and upon acceptance by ANGLISTICUM for publication of the manuscript in the Journal, I/We hereby assign and transfer publication rights to ANGLISTICUM, whereas I/We retain the copyright for the manuscript. This assignment provides ANGLISTICUM the sole right and responsibility to publish the manuscript in its printed and online version, and/or in other media formats.
2. In consideration of this assignment, ANGLISTICUM hereby undertakes to prepare and publish the manuscript in the Journal, subject only to its right to refuse publication if there is a breach of the Author’s warranty in paragraph 4 or if there are other reasonable grounds.
3. Editors and the editorial board of ANGLISTICUM are empowered to make such editorial changes as may be necessary to make the Manuscript suitable for publication.
4. I/We hereby acknowledge that: (a) The manuscript submitted is an original work and that I/We participated in the work substantively and thus I/We hereby are prepared to take public responsibility for the work; (b) I/We hereby have seen and approved the manuscript as submitted and that the manuscript has not either been published, submitted or considered for publication elsewhere; (c) The text, illustration, and any other materials included in the manuscript do not infringe upon any existing copyright or other rights of anyone.
5. I/We hereby indemnify ANGLISTICUM and the respective Editors of the Journal as mentioned in paragraph 3, and hold them harmless from any loss, expense or damage occasioned by a claim or suit by a third party for copyright infringement, or any suit arising out of any breach of the foregoing warranties as a result of publication of the manuscript.
